Shanxian Chemical
SUPPLEMENTS
Enzyme Lab Liver Hydrogen Peroxide
Enzyme experiment: liver and hydrogen peroxide
Experimental purpose
To explore the role of enzymes in the liver on the decomposition of hydrogen peroxide.
Experimental materials
Fresh liver, hydrogen peroxide solution, mortar, test tube, dropper, health incense, etc.
Experimental steps
1. ** Preparation of liver grinding liquid **: Take an appropriate amount of fresh liver, place it in a mortar, add a small amount of quartz sand and an appropriate amount of distilled water, after fully grinding, filter it with filter paper to collect the filtrate, which is the liver grinding liquid, which is rich in catalase.
2. ** Experimental grouping and operation **:
- ** Group 1 **: Take a clean test tube, add 2mL of hydrogen peroxide solution, put the sanitary incense with Mars in the test tube mouth, and observe the burning of the sanitary incense. At this time, hydrogen peroxide decomposes slowly at room temperature, produces less oxygen, and there is no obvious sign of reignition of the sanitary incense.
- * Group 2 **: Take another test tube, add 2mL of hydrogen peroxide solution, and heat it with an alcohol lamp water bath. As the temperature increases, the decomposition rate of hydrogen peroxide accelerates, and more bubbles are generated. Put the sanitary incense with Mars in the test tube mouth, and the sanitary incense reignites, indicating that heating can accelerate the decomposition of hydrogen peroxide and produce oxygen.
- ** Third group **: Take another test tube, add 2mL of hydrogen peroxide solution, and then add a few drops of liver grinding liquid dropwise. A large number of bubbles pop up violently in an instant, and the sanitary incense with Mars is placed at the mouth of the test tube, and the sanitary incense is violently reignited. This shows that the catalase in the liver grinding liquid can efficiently catalyze the decomposition of hydrogen peroxide and produce a large amount of oxygen.
Experimental conclusions
1. The enzyme is highly efficient. Compared with heating, the catalase in the liver can accelerate the decomposition rate of hydrogen peroxide more significantly. The effect of catalase on reducing the activation energy of chemical reactions is more significant than that of heating, so that hydrogen peroxide can be rapidly decomposed into water and oxygen under mild conditions.
2. This experiment intuitively demonstrates that enzymes in organisms act as high-efficiency catalysts, which play a key catalytic role in various chemical reactions in metabolism and maintain the normal progress of life activities.
Experimental purpose
To explore the role of enzymes in the liver on the decomposition of hydrogen peroxide.
Experimental materials
Fresh liver, hydrogen peroxide solution, mortar, test tube, dropper, health incense, etc.
Experimental steps
1. ** Preparation of liver grinding liquid **: Take an appropriate amount of fresh liver, place it in a mortar, add a small amount of quartz sand and an appropriate amount of distilled water, after fully grinding, filter it with filter paper to collect the filtrate, which is the liver grinding liquid, which is rich in catalase.
2. ** Experimental grouping and operation **:
- ** Group 1 **: Take a clean test tube, add 2mL of hydrogen peroxide solution, put the sanitary incense with Mars in the test tube mouth, and observe the burning of the sanitary incense. At this time, hydrogen peroxide decomposes slowly at room temperature, produces less oxygen, and there is no obvious sign of reignition of the sanitary incense.
- * Group 2 **: Take another test tube, add 2mL of hydrogen peroxide solution, and heat it with an alcohol lamp water bath. As the temperature increases, the decomposition rate of hydrogen peroxide accelerates, and more bubbles are generated. Put the sanitary incense with Mars in the test tube mouth, and the sanitary incense reignites, indicating that heating can accelerate the decomposition of hydrogen peroxide and produce oxygen.
- ** Third group **: Take another test tube, add 2mL of hydrogen peroxide solution, and then add a few drops of liver grinding liquid dropwise. A large number of bubbles pop up violently in an instant, and the sanitary incense with Mars is placed at the mouth of the test tube, and the sanitary incense is violently reignited. This shows that the catalase in the liver grinding liquid can efficiently catalyze the decomposition of hydrogen peroxide and produce a large amount of oxygen.
Experimental conclusions
1. The enzyme is highly efficient. Compared with heating, the catalase in the liver can accelerate the decomposition rate of hydrogen peroxide more significantly. The effect of catalase on reducing the activation energy of chemical reactions is more significant than that of heating, so that hydrogen peroxide can be rapidly decomposed into water and oxygen under mild conditions.
2. This experiment intuitively demonstrates that enzymes in organisms act as high-efficiency catalysts, which play a key catalytic role in various chemical reactions in metabolism and maintain the normal progress of life activities.
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