Shanxian Chemical
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Triethylamine in HPLC Mobile Phase
On the Application of Triethylamine in Mobile Phase of High Performance Liquid Chromatography
Husband High Performance Liquid Chromatography is also a tool for separation and analysis. The mobile phase in between is like water in a boat, which is very important. Triethylamine often has unique uses in mobile phases of high performance liquid chromatography.
Triethylamine, active. In the mobile phase, it can interact with many substances. First, it can adjust the pH of the mobile phase. Cover the separation of high performance liquid chromatography, often depends on the chemical properties of the material. Changes in pH can make the dissociation state of the substance to be separated different, which in turn affects its role with the stationary phase, and ultimately improves the separation effect.
Furthermore, triethylamine can be combined with some easily ionized compounds. In this way, the interaction between such compounds and the residual silanol group on the surface of the stationary phase can be reduced. The capping silanol group is easy to cause peak tailing, and the addition of triethylamine can effectively inhibit this phenomenon, make the chromatographic peak more sharp and symmetrical, and improve the accuracy of detection.
However, the use of triethylamine also needs to be cautious. Excessive dosage may cause drastic changes in the properties of the mobile phase and affect the reproducibility of separation. And triethylamine has a certain volatility and odor. When operating, pay attention to ventilation to ensure the safety of the experimental environment.
To be sure, triethylamine is a double-edged sword in the mobile phase of high performance liquid chromatography. Good use of it can improve the separation efficiency and help analysis and testing; improper use, it is prone to many drawbacks. Experimenters should explore in detail and weigh the pros and cons in order to achieve the best experimental effect.
Husband High Performance Liquid Chromatography is also a tool for separation and analysis. The mobile phase in between is like water in a boat, which is very important. Triethylamine often has unique uses in mobile phases of high performance liquid chromatography.
Triethylamine, active. In the mobile phase, it can interact with many substances. First, it can adjust the pH of the mobile phase. Cover the separation of high performance liquid chromatography, often depends on the chemical properties of the material. Changes in pH can make the dissociation state of the substance to be separated different, which in turn affects its role with the stationary phase, and ultimately improves the separation effect.
Furthermore, triethylamine can be combined with some easily ionized compounds. In this way, the interaction between such compounds and the residual silanol group on the surface of the stationary phase can be reduced. The capping silanol group is easy to cause peak tailing, and the addition of triethylamine can effectively inhibit this phenomenon, make the chromatographic peak more sharp and symmetrical, and improve the accuracy of detection.
However, the use of triethylamine also needs to be cautious. Excessive dosage may cause drastic changes in the properties of the mobile phase and affect the reproducibility of separation. And triethylamine has a certain volatility and odor. When operating, pay attention to ventilation to ensure the safety of the experimental environment.
To be sure, triethylamine is a double-edged sword in the mobile phase of high performance liquid chromatography. Good use of it can improve the separation efficiency and help analysis and testing; improper use, it is prone to many drawbacks. Experimenters should explore in detail and weigh the pros and cons in order to achieve the best experimental effect.
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